| Autor: |
Novaes E Brito RR; Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de São Paulo , Brazil ., Xander P, Pérez EC, Maricato JT, Laurindo MF, De Lorenzo BH, Pellegrino R, Bernardo V, Lopes JD, Mariano M |
| Jazyk: |
angličtina |
| Zdroj: |
Immunological investigations [Immunol Invest] 2014; Vol. 43 (7), pp. 675-92. Date of Electronic Publication: 2014 Jun 20. |
| DOI: |
10.3109/08820139.2014.915413 |
| Abstrakt: |
New Zealand Black X New Zealand White F1 [(NZB/NZW)F1] mice develop an autoimmune condition with similarities to human systemic lupus erythematosus (SLE). In this study, we demonstrate that B-1 cells, which have previously been reported to be involved in several autoimmune diseases, have altered gene expression in these mice. RNA was extracted from purified B-1 cells of disease-free C57BL/6 mice and lupus-prone (NZB/NZW)F1 mice. Gene expression was analysed using DNA microarray techniques and validated by real time reverse transcriptase polymerase chain reaction (RT-PCR). In (NZB/NZW)F1 mice, some genes had altered expression patterns compared to disease-free controls. Specifically, the upregulation of Ifitm1, Pvrl2 and Ifi202b and downregulation of Trp53bp1 mRNA were observed in (NZB/NZW)F1 mice. These genes are known to be associated with autoimmune diseases. This pattern of gene expression in B-1 cells could understanding of the pathogenesis of SLE. Thus, it is reasonable to hypothesise that the altered gene expression observed in B-1 cells in our experimental model is important for SLE prognosis and therapy, and these implications are discussed herein. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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