| Autor: |
do Rosário TR; Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São Paulo SP Brazil., Dib CC; Laboratório de Tuberculose Centro de Pesquisa e Desenvolvimento de Sanidade Animal Instituto Biológico de São Paulo São PauloSP Brazil., Roxo E; Laboratório de Tuberculose Centro de Pesquisa e Desenvolvimento de Sanidade Animal Instituto Biológico de São Paulo São PauloSP Brazil., Pinheiro SR; Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São Paulo SP Brazil., Vasconcellos SA; Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São Paulo SP Brazil., Benites NR; Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São Paulo SP Brazil. |
| Jazyk: |
angličtina |
| Zdroj: |
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2014 May 02; Vol. 45 (1), pp. 225-30. Date of Electronic Publication: 2014 May 02 (Print Publication: 2014). |
| DOI: |
10.1590/S1517-83822014005000038 |
| Abstrakt: |
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12(nd) and 25(th) day of culture). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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