| Autor: |
Chah KF; Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka ., Gómez-Sanz E; Biochemistry and Molecular Biology University of La Rioja Logroño Spain., Nwanta JA; Department of Veterinary Public Health and Preventive Medicine University of Nigeria Nsukka ., Asadu B; Department of Veterinary Public Health and Preventive Medicine University of Nigeria Nsukka ., Agbo IC; Department of Veterinary Pathology and Microbiology University of Nigeria Nsukka ., Lozano C; Biochemistry and Molecular Biology University of La Rioja Logroño Spain., Zarazaga M; Biochemistry and Molecular Biology University of La Rioja Logroño Spain., Torres C; Biochemistry and Molecular Biology University of La Rioja Logroño Spain. |
| Abstrakt: |
The occurrence, resistance phenotype and molecular mechanisms of resistance of methicillin-resistant staphylococci from groin swabs of 109 clinically healthy dogs in Nsukka, Nigeria were investigated. The groin swab samples were cultured on mannitol salt agar supplemented with 10 μg of cloxacillin. Sixteen methicillin-resistant coagulase negative staphylococci (MRCoNS), all harbouring the mecA gene were isolated from 14 (12.8%) of the 109 dogs studied. The MRCoNS isolated were: S. sciuri subspecies rodentium, S. lentus, S. haemolyticus, and S. simulans with S. sciuri subspecies rodentium (62.5%) being the predominant species. Thirteen (81.3%) of the MRCoNS were resistant to tetracycline while 12 (75%) and 10 (62.5%) were resistant to kanamycin and trimthoprim-sulphamethoxazole respectively. None of the isolates was resistant to fusidic acid, linezolid and vancomycin. Thirteen (81.3%) of the MRCoNS were multi-drug resistance (MDR). Other antimicrobial genes detected were: blaZ, tet(K), tet(M), tet(L), erm(B), lnu(A), aacA-aphD, aphA3, str, dfr(G), cat pC221 , and cat pC223 . Methicillin-resistant staphylococci are common colonizers of healthy dogs in Nigeria with a major species detected being S. sciuri subsp. rodentium. |
