Antibiogram and plasmid profiling of carbapenemase and extended spectrum Beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae in Abeokuta, South western, Nigeria.

Autor:	Motayo BO; Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria., Akinduti PA; Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria., Adeyakinu FA; Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria., Okerentugba PO; Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria., Nwanze JC; Department of Pharmacology and Therapeutics, Igbinedion University, Okada, Edo State, Nigeria., Onoh CC; Department of Pharmacology and Therapeutics, Igbinedion University, Okada, Edo State, Nigeria., Innocent-Adiele HC; Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria., Okonko IO; Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria.
Jazyk:	angličtina
Zdroj:	African health sciences [Afr Health Sci] 2013 Dec; Vol. 13 (4), pp. 1091-7.
DOI:	10.4314/ahs.v13i4.33
Abstrakt:	Background: The increased reports of ESBL dissemination from various centres in south western, Nigeria and the recent emergence of carbapenem resistant bacteria prompted the conception of this study. Objectives: To demonstrate the relationship between high molecular weight plasmids and the expression of antibiotic multi-resistance including ESBL and carbapenemase. Methods: We investigated 97 isolates of selected organisms consisting of 67 E. coli and 30 Klebseilla spp for the presence of plasmids expressing ESBL including carbapenem-hydrolysing enzymes. Beta-lactamase was determined using acidometric method, while ESBL and carbapenemase activity was determined using the double-disk diffusion test as well as the Modified Hodge test (MHT). Plasmid profiles of ESBL and carbapenemase positive isolates were determined according to standard protocols. Results: An ESBL prevalence rate of 21.6% and carbapenem- resistance rate of 9.3% was recorded. Antibiotic susceptibility profile of ESBL isolates showed 100.0% resistance against Amoxicillin, Cotrimoxazole and Erythromycin. Moderate susceptibility was recorded against the Quinolone class of antibiotics; Meropenem remained the most active antibiotic against ESBL isolates with 62.5% against E. coli and 60% against K. pneumoniae. The plasmid profiles of our study isolates ranged from 11.8kbp to 35.5kbp. Conclusion: Due to the relationship between high molecular weight plasmids and multi-drug resistance, we hereby recommend regular molecular surveillance of this form in our study setting.
Databáze:	MEDLINE
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