Preventing adhesion of Escherichia coli O157:H7 and Salmonella Typhimurium LT2 on tomato surfaces via ultrathin polyethylene glycol film.

Autor: Zhang M; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122., Yang F; Department of Chemistry, Texas A&M University, College Station, Texas 77943-3144., Pasupuleti S; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122., Oh JK; Department of Materials Science and Engineering, Texas A&M University, College Station, TX 77843-3003., Kohli N; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122., Lee IS; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122., Perez K; Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843-2253., Verkhoturov SV; Department of Chemistry, Texas A&M University, College Station, Texas 77943-3144., Schweikert EA; Department of Chemistry, Texas A&M University, College Station, Texas 77943-3144., Jayaraman A; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122., Cisneros-Zevallos L; Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-3244., Akbulut M; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122; Department of Materials Science and Engineering, Texas A&M University, College Station, TX 77843-3003. Electronic address: makbulut@tamu.ed.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2014 Aug 18; Vol. 185, pp. 73-81. Date of Electronic Publication: 2014 Jun 02.
DOI: 10.1016/j.ijfoodmicro.2014.05.019
Abstrakt: This work deals with adhesion of Escherichia coli O157:H7 and Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S. Typhimurium LT2) on polyethylene glycol (PEG) coated tomato surfaces. PEG coating was characterized by water contact angle technique, scanning electron microscopy, and secondary ion mass spectrometry. It was shown that PEG films could physisorb on the tomato surfaces after the oxygen plasma treatment, which made some outermost layers of the surfaces hydrophilic. Bacterial adhesion on PEG coated tomato surface was studied by standard plate count, fluorescence microscopy, and scanning electron microscopy techniques. Fully covered PEG film reduced the bacterial attachment 90% or more in comparison to the bare tomato surface. The degree of bacterial attachment decreased exponentially with increasing PEG coverage. When desired, PEG film could be removed by rinsing with water. Overall, this work demonstrates the proof-of-concept that an ultrathin film of polyethylene glycol may be used to effectively inhibit the attachment of pathogenic bacteria on tomato surfaces.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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