Pyrrolidine dithiocarbamate inhibits UVB-induced skin inflammation and oxidative stress in hairless mice and exhibits antioxidant activity in vitro.

Autor: Ivan AL; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: analauraivan@hotmail.com., Campanini MZ; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: marcelazbm@hotmail.com., Martinez RM; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: renatafarma_55@yahoo.com.br., Ferreira VS; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: vsottero@hotmail.com., Steffen VS; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: vsteffen@gmail.com., Vicentini FT; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Av. do Café s/n, 14049-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: fabianatmcv@hotmail.com., Vilela FM; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Av. do Café s/n, 14049-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: nandajm@fcfrp.usp.br., Martins FS; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Av. do Café s/n, 14049-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: fsmfarmacia@hotmail.com., Zarpelon AC; Departamento de Patologia, Universidade Estadual de Londrina-UEL, Rod. Celso Garcia Cid, Km 380, PR 445, Cx. Posta 10.001, 86057-970 Londrina, Paraná, Brazil. Electronic address: ana.zarpelon@hotmail.com., Cunha TM; Departamento de Farmacologia, Faculdade de Medicina de Ribeirao Preto-USP, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, São Paulo, Brazil. Electronic address: thicunha@usp.br., Fonseca MJ; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Av. do Café s/n, 14049-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: magika@fcfrp.usp.br., Baracat MM; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: baracat1903@yahoo.com.br., Georgetti SR; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: sangeorgetti@gmail.com., Verri WA Jr; Departamento de Patologia, Universidade Estadual de Londrina-UEL, Rod. Celso Garcia Cid, Km 380, PR 445, Cx. Posta 10.001, 86057-970 Londrina, Paraná, Brazil. Electronic address: waldiceujr@yahoo.com.br., Casagrande R; Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina Londrina-UEL, Hospital Universitário, Avenida Robert Koch, 60, 86039-440 Londrina, Paraná, Brazil. Electronic address: rubiacasa@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2014 Sep 05; Vol. 138, pp. 124-33. Date of Electronic Publication: 2014 May 27.
DOI: 10.1016/j.jphotobiol.2014.05.010
Abstrakt: Ultraviolet B (UVB) irradiation may cause oxidative stress- and inflammation-dependent skin cancer and premature aging. Pyrrolidine dithiocarbamate (PDTC) is an antioxidant and inhibits nuclear factor-κB (NF-κB) activation. In the present study, the mechanisms of PDTC were investigated in cell free oxidant/antioxidant assays, in vivo UVB irradiation in hairless mice and UVB-induced NFκB activation in keratinocytes. PDTC presented the ability to scavenge 2,2'-azinobis-(3-ethyl benzothiazoline-6-sulfonic acid) radical (ABTS), 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH) and hydroxyl radical (OH); and also efficiently inhibited iron-dependent and -independent lipid peroxidation as well as chelated iron. In vivo, PDTC treatment significantly decreased UVB-induced skin edema, myeloperoxidase (MPO) activity, production of the proinflammatory cytokine interleukin-1β (IL-1β), matrix metalloproteinase-9 (MMP-9), increase of reduced glutathione (GSH) levels and antioxidant capacity of the skin tested by the ferric reducing antioxidant power (FRAP) and ABTS assays. PDTC also reduced UVB-induced IκB degradation in keratinocytes. These results demonstrate that PDTC presents antioxidant and anti-inflammatory effects in vitro, which line up well with the PDTC inhibition of UVB irradiation-induced skin inflammation and oxidative stress in mice. These data suggest that treatment with PDTC may be a promising approach to reduce UVB irradiation-induced skin damages and merits further pre-clinical and clinical studies.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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