Myelo-enhancement by astragalus membranaceus in male albino rats with chemotherapy myelo-suppression. Histological and immunohistochemical study.

Autor:	Ismail ZM; Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt., Amin NM; Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt., Yacoub MF; Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt., Mohamed AM; Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Jazyk:	angličtina
Zdroj:	International journal of stem cells [Int J Stem Cells] 2014 May; Vol. 7 (1), pp. 12-22.
DOI:	10.15283/ijsc.2014.7.1.12
Abstrakt:	Background and Objectives: Myelosuppression is the most common toxicity encountered in the oncology clinic today. This study was planned to investigate the possible protective and therapeutic role of the traditional Chinese Medicinal Herb; Astragalus Membranaceus (AM), on chemotherapy-induced myelosuppression. Methods and Results: This study was carried out on thirty six adult male albino rats. They were divided into: Group I Control Group (n=6) received a vehicle of phosphate buffered saline (PBS) solution. Group II (n=12) were injected I.P. with cyclophosphamide (CY) for 3 days (gIIa n =6) and continued for one more week to receive AM orally (gIIb n=6). Group III (n=6) received CY I.P. together with AM orally for 3 days. Group IV (n=12) received AM orally for one week (gIVa n=6) and continued for extra three days receiving CY I.P. with AM orally (gIVb n=6). Blood samples were analysed for Total Leucocytic Count and Lymphocytic Count. Counting of CD34 +ve cells in bone marrow was performed by flowcytometry. Bone marrow sections were subjected to H&E stain as well as immunohistochemical staining for anti- CD20 antibody. The mean area % of cellular bone marrow regions occupied by developing haemopoietic cells, mean area of fat cells and mean number of CD20 immunopositive B lymphocytes in the bone marrow were measured by histomorphometric studies and statistically compared. AM proved to have a myelo-protective and myelo-therapeutic capacity, evidenced at both laboratory and morphological levels. Conclusions: The greatest myelo-potentiating effect of AM was achieved when supplied before and together with CY therapy.
Databáze:	MEDLINE
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