Enzyme-linked immunosorbent assay (ELISA) for the detection of use of the synthetic cannabinoid agonists UR-144 and XLR-11 in human urine.

Autor:	Mohr AL; The Center for Forensic Science Research and Education, Willow Grove, PA, USA., Ofsa B; NMS Labs, Willow Grove, PA 18901, USA., Keil AM; NMS Labs, Willow Grove, PA 18901, USA., Simon JR; Tulip Biolabs, Inc., West Point, PA 19486, USA., McMullin M; NMS Labs, Willow Grove, PA 18901, USA., Logan BK; The Center for Forensic Science Research and Education, Willow Grove, PA, USA NMS Labs, Willow Grove, PA 18901, USA barry.logan@nmslabs.com.
Jazyk:	angličtina
Zdroj:	Journal of analytical toxicology [J Anal Toxicol] 2014 Sep; Vol. 38 (7), pp. 427-31. Date of Electronic Publication: 2014 Jun 07.
DOI:	10.1093/jat/bku049
Abstrakt:	Ongoing changes in the synthetic cannabinoid drug market create the need for relevant targeted immunoassays for rapid screening of biological samples. We describe the validation and performance characteristics of an enzyme-linked immunosorbent assay designed to detect use of one of the most prevalent synthetic cannabinoids in urine, UR-144, by targeting its pentanoic acid metabolite. Fluorinated UR-144 (XLR-11) has been demonstrated to metabolize to this common product. The assay has significant cross-reactivity with UR-144-5-OH, UR-144-4-OH and XLR-11-4-OH metabolites, but <10% cross-reactivity with the parent compounds, and no measurable cross-reactivity with other synthetic cannabinoids and their metabolites at concentrations of <1,000 ng/mL. The assay's cutoff is 5 ng/mL relative to the pentanoic acid metabolite of UR-144, which is used as the calibrator. The method was validated with 90 positive and negative control urine samples for UR-144, XLR-11 and its metabolites tested versus liquid chromatography-tandem mass spectrometry. The accuracy, sensitivity and specificity were determined to be 100% for the assay at the specified cutoff. (© The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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