Is prediffusion test an alternative to improve accuracy in screening hVISA strains and to detect susceptibility to glycopeptides/lipopeptides?

Autor: Silveira AC; Federal University of Health Sciences of Porto Alegre, RS, Brazil; University Regional of Blumenau, SC, Brazil. Electronic address: alessandrocosilveira@gmail.com., Sambrano GE; Federal University of Health Sciences of Porto Alegre, RS, Brazil., Paim TG; Federal University of Health Sciences of Porto Alegre, RS, Brazil., Caierão J; Federal University of Health Sciences of Porto Alegre, RS, Brazil., Cordova CM; University Regional of Blumenau, SC, Brazil., d'Azevedo PA; Federal University of Health Sciences of Porto Alegre, RS, Brazil.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2014 Aug; Vol. 79 (4), pp. 401-4. Date of Electronic Publication: 2014 May 09.
DOI: 10.1016/j.diagmicrobio.2014.04.008
Abstrakt: The characterization of heteroresistant vancomycin-intermediate Staphylococcus aureus strains (hVISA) is even more challenging, as no routine standardized laboratory methods are available. A total of 124 S. aureus isolates recovered from inpatients attended in hospitals of Santa Catarina State, Southern Brazil, were evaluated. The MIC of vancomycin, teicoplanin, and daptomycin was determined by Etest and prediffusion tests using NeoSensitabs® tablets. All isolates were susceptible to vancomycin (MICs: 0.5-3 μg/mL) by Etest. However, according to prediffusion test, 17 isolates presented reduced susceptibility to vancomycin, and of these, 12 were confirmed as hVISA using populational analysis. Considering daptomycin, prediffusion results were in agreement with susceptibility data (MICs), as all isolates were susceptible. Considering that characterizing hVISA is challenging and that MIC determination is not adequate to characterize this phenotype, prediffusion test was a viable alternative to screening hVISA and reduced susceptibility to vancomycin. It was simple and low cost, with accuracy comparable to other well-established methods.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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