Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries.

Autor: Chae JJ; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Mulreany DG; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Guo Q; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Lu Q; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Choi JS; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Strehin I; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Espinoza FA; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287., Schein O; Department of Ophthalmology, Johns Hopkins University, 600 N. Wolfe Street, Baltimore, MD 21287., Trexler MM; Research and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20723., Bower KS; Department of Ophthalmology, Johns Hopkins University, 600 N. Wolfe Street, Baltimore, MD 21287., Elisseeff JH; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, 400 N. Wolfe Street, Baltimore, MD 21287.
Jazyk: angličtina
Zdroj: Military medicine [Mil Med] 2014 Jun; Vol. 179 (6), pp. 686-94.
DOI: 10.7205/MILMED-D-13-00360
Abstrakt: This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.
(Reprint & Copyright © 2014 Association of Military Surgeons of the U.S.)
Databáze: MEDLINE
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