Gene disruption reveals a dispensable role for plasmepsin VII in the Plasmodium berghei life cycle.

Autor: Mastan BS; Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India., Kumari A; Bioinformatics Laboratory, SCB Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India., Gupta D; Bioinformatics Laboratory, SCB Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India., Mishra S; Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, India., Kumar KA; Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India. Electronic address: kaksl@uohyd.ernet.in.
Jazyk: angličtina
Zdroj: Molecular and biochemical parasitology [Mol Biochem Parasitol] 2014 Jun; Vol. 195 (1), pp. 10-3. Date of Electronic Publication: 2014 Jun 02.
DOI: 10.1016/j.molbiopara.2014.05.004
Abstrakt: Plasmepsins (PM), aspartic proteases of Plasmodium, comprises a family of ten proteins that perform critical functions in Plasmodium life cycle. Except VII and VIII, functions of the remaining plasmepsin members have been well characterized. Here, we have generated a mutant parasite lacking PM VII in Plasmodium berghei using reverse genetics approach. Systematic comparison of growth kinetics and infection in both mosquito and vertebrate host revealed that PM VII depleted mutants exhibited no defects in development and progressed normally throughout the parasite life cycle. These studies suggest a dispensable role for PM VII in Plasmodium berghei life cycle.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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