Detection of stealthy small amphiphilic biomarkers.

Autor: Sakamuri RM; Chemistry Division, MS J567, Los Alamos National Laboratory, Los Alamos, NM 87544, United States., Capek P; Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, United States., Dickerson TJ; Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, United States., Barry CE 3rd; Tuberculosis Research Section, National Institute of Allergy and Infectious Disease, Bethesda MD, United States., Mukundan H; Chemistry Division, MS J567, Los Alamos National Laboratory, Los Alamos, NM 87544, United States. Electronic address: harshini@lanl.gov., Swanson BI; Chemistry Division, MS J567, Los Alamos National Laboratory, Los Alamos, NM 87544, United States. Electronic address: basil@lanl.gov.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2014 Aug; Vol. 103, pp. 112-7. Date of Electronic Publication: 2014 May 28.
DOI: 10.1016/j.mimet.2014.05.012
Abstrakt: Pathogen-specific biomarkers are secreted in the host during infection. Many important biomarkers are not proteins but rather small molecules that cannot be directly detected by conventional methods. However, these small molecule biomarkers, such as phenolic glycolipid-I (PGL-I) of Mycobacterium leprae and Mycobactin T (MbT) of Mycobacterium tuberculosis, are critical to the pathophysiology of infection, and may be important in the development of diagnostics, vaccines, and novel therapeutic strategies. Methods for the direct detection of these biomarkers may be of significance both for the diagnosis of infectious disease, and also for the laboratory study of such molecules. Herein, we present, for the first time, a transduction approach for the direct and rapid (30min) detection of small amphiphilic biomarkers in complex samples (e.g. serum) using a single affinity reagent. To our knowledge, this is the first demonstration of an assay for the direct detection of PGL-I, and the first single-reporter assay for the detection of MbT. The assay format exploits the amphiphilic chemistry of the small molecule biomarkers, and is universally applicable to all amphiphiles. The assay is only the first step towards developing a robust system for the detection of amphiphilic biomarkers that are critical to infectious disease pathophysiology.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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