| Autor: |
Piacentini N; Laboratory of Cell Biophysics, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. niccolo.piacentini@epfl.ch., Verkhovsky AB, Gabella C, Meister JJ, Vianay B |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2014 Jul 21; Vol. 14 (14), pp. 2539-47. |
| DOI: |
10.1039/c4lc00188e |
| Abstrakt: |
Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far difficult to access in a living cell. Here we propose a micro-fabricated two-component setup for direct tension measurement on a peripheral bundle within an intact cell. We used 3-D substrates made of silicon elastomer to elevate the cell making the filament bundle at its border accessible from the side, and employed an ultra-soft (spring constant 0.78 nN μm(-1)) epoxy-based cantilever for mechanical probing. With this setup we were able for the first time to measure the tension in peripheral actin bundles in living primary fibroblasts spread on a rigid substrate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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