Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever.

Autor: Russell GC; Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK. Electronic address: george.russell@moredun.ac.uk., Scholes SF; Animal Health and Veterinary Laboratories Agency (AHVLA), Pentlands Science Park, Midlothian, UK., Twomey DF; AHVLA Starcross Regional Laboratory, Staplake Mount, Starcross, Devon EX6 8PE, UK., Courtenay AE; AHVLA Surveillance Centre, Division of Livestock Health and Welfare, School of Veterinary Science, Leahurst Campus, University of Liverpool, Wirral CH64 7TE, UK., Grant DM; Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK., Lamond B; Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK., Norris D; Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK., Willoughby K; Moredun Scientific, Pentlands Science Park, Penicuik EH26 0PZ, UK., Haig DM; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK., Stewart JP; Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park IC2, L3 5RF, UK.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2014 Aug 06; Vol. 172 (1-2), pp. 63-71. Date of Electronic Publication: 2014 Apr 28.
DOI: 10.1016/j.vetmic.2014.04.011
Abstrakt: In order to define better virus isolates from animals with malignant catarrhal fever (MCF), segments of three genes of ovine herpesvirus-2 were amplified from diagnostic samples representing MCF cases with a range of clinical presentations in cattle, including head and eye, alimentary and neurological. The variation within each gene segment was estimated by DNA sequencing, which confirmed that the newly-annotated Ov9.5 gene was significantly more polymorphic than either of the other loci tested (segments of ORF50 and ORF75), with alleles that differed at over 60% of nucleotide positions. Despite this, the nine Ov9.5 alleles characterised had identical predicted splicing patterns and could be translated into Ov9.5 polypeptides with at least 49% amino acid identity. This multi-locus approach has potential for use in epidemiological studies and in charactering chains of infection. However there was no association between specific variants of OvHV-2 and the clinical/pathological presentation of MCF in the cattle analysed.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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