Glutathione peroxidase 3 serum levels and GPX3 gene polymorphisms in subjects with metabolic syndrome.

Autor: Baez-Duarte BG; Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), México., Mendoza-Carrera F; Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México., García-Zapién A; Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México., Flores-Martínez SE; Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México., Sánchez-Corona J; Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México., Zamora-Ginez I; Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), México., Torres-Rasgado E; Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), México., León-Chávez BA; Facultad de Ciencias Químicas, BUAP, Puebla, México., Pérez-Fuentes R; Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), México; Centro de Investigación Biomédica de Oriente (CIBIOR), IMSS, Atlixco, Puebla, México. Electronic address: rycardoperez@hotmail.com.
Jazyk: angličtina
Zdroj: Archives of medical research [Arch Med Res] 2014 Jul; Vol. 45 (5), pp. 375-82. Date of Electronic Publication: 2014 May 10.
DOI: 10.1016/j.arcmed.2014.05.001
Abstrakt: Background and Aims: Glutathione peroxidase 3 (GPx3) plays a main role in removing hydro- and lipoperoxides from the body. Changes in concentration and several single-nucleotide polymorphisms (SNP) at the GPX3 gene have been associated with vascular diseases, but the relationship of GPx3 with metabolic syndrome (MetS) remains unexplored. We undertook this study to determine the association of GPx3 serum levels and several GPX3 SNPs with the presence of MetS in Mexican subjects.
Methods: Clinical, biochemical, and anthropometric evaluation were conducted in 426 subjects assigned to three groups: control (n = 42); risk group (RG, n = 200), and MetS group (n = 184). Insulin sensitivity (IS) and cardiovascular risk were determined by the QUICKI and TG/HDL-C index, respectively. Serum GPx3 was determined by enzyme immunoassay and polymorphisms within GPX3 gene were identified by nucleotide sequencing.
Results: MetS group showed low IS and increased cardiovascular risk with respect to controls as well as higher GPx3 serum levels (172.9 ± 32.2 vs. 145.6 ± 24.8 ng/dL; p <0.05). Only three of the ten GPX3 SNPs screened were polymorphic with two haplotypes observed (CCT and TTA-rs8177404, rs8177406, and rs8177409), indicating tight linkage disequilibrium in this genetic region. No differences for either genotype or allele frequencies among groups were observed, but rs8177409 (allele T) was associated with cardiovascular risk (odds ratio [OR], 4.5; p = 0.0125).
Conclusion: This study shows that serum levels of GPx3 are increased in subjects with MetS and that rs8177409 SNP was associated with cardiovascular risk in a Mexican population.
(Copyright © 2014 IMSS. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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