Expression of stabilin-1 in M2 macrophages in human granulomatous disease and melanocytic lesions.

Autor:	Schönhaar K; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Schledzewski K; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Michel J; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Dollt C; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Gkaniatsou C; Department of Internal Medicine, City Hospital Baden-Baden Balger Str. 50, 76532 Baden-Baden, Germany., Géraud C; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Kzhyshkowska J; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Goerdt S; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany., Schmieder A; Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatoloy T. Kutzer Ufer 1-3, 68167 Mannheim, Germany.
Jazyk:	angličtina
Zdroj:	International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2014 Mar 15; Vol. 7 (4), pp. 1625-34. Date of Electronic Publication: 2014 Mar 15 (Print Publication: 2014).
Abstrakt:	Stabilin-1 is an endocytotic scavenger receptor, specifically expressed by non-continuous sinusoidal endothelial cells in the liver, spleen and lymph nodes and by M2 or alternatively activated macrophages in human malignancies. We analysed paraffin-embedded tissue of melanocytic lesions and granulomatous diseases for stabilin-1 expression, using the human/murine RS1 antibody. The specificity of the RS1 staining was confirmed in a knockout model, as only M2-like tumor-associated macrophages and vessels of a B16F10 melanoma in wild type mice stained positive; while staining of tumor-associated macrophages and vessels originating from stabilin-1 deficient mice remained negative for stabilin-1 specific antibody RS1. In human specimens, the RS1 antibody stained tumor-associated macrophages in all pathological stages of melanoma. In addition, five cases of juvenile xanthogranulomas and one case of necrobiotic xanthogranuloma were strongly stabilin-1 positive, while Th-1 cytokine dominated granulomatous diseases such as sarcoidosis and granulomatous leprosy were negative. Stabilin-1 positive vessels were found in all analysed non-Langerhans cell histiocytoses and melanocytic lesions. No stabilin-1 positive vessels were present in any other granulomatous diseases.
Databáze:	MEDLINE
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