Feline fecal virome reveals novel and prevalent enteric viruses.

Autor: Ng TF; Blood Systems Research Institute, San Francisco, San Francisco, CA, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA., Mesquita JR; Department of Animal Science, Rural Engineering and Veterinary, Polytechnic Institut of Viseu, Viseu, Portugal., Nascimento MS; Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal., Kondov NO; Blood Systems Research Institute, San Francisco, San Francisco, CA, USA., Wong W; Blood Systems Research Institute, San Francisco, San Francisco, CA, USA., Reuter G; Regional Laboratory of Virology, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary., Knowles NJ; The Pirbright Institute, Woking, Surrey, United Kingdom., Vega E; NCIRD, National Calicivirus Laboratory, Centers for Disease Control and Prevention, Atlanta, GA, USA., Esona MD; GRVLB, Rotavirus Surveillance, Centers for Disease Control and Prevention, Atlanta, GA, USA., Deng X; Blood Systems Research Institute, San Francisco, San Francisco, CA, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA., Vinjé J; NCIRD, National Calicivirus Laboratory, Centers for Disease Control and Prevention, Atlanta, GA, USA., Delwart E; Blood Systems Research Institute, San Francisco, San Francisco, CA, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA. Electronic address: delwarte@medicine.ucsf.edu.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2014 Jun 25; Vol. 171 (1-2), pp. 102-11. Date of Electronic Publication: 2014 Apr 13.
DOI: 10.1016/j.vetmic.2014.04.005
Abstrakt: Humans keep more than 80 million cats worldwide, ensuring frequent exposure to their viruses. Despite such interactions the enteric virome of cats remains poorly understood. We analyzed a fecal sample from a single healthy cat from Portugal using viral metagenomics and detected five eukaryotic viral genomes. These viruses included a novel picornavirus (proposed genus "Sakobuvirus") and bocavirus (feline bocavirus 2), a variant of feline astrovirus 2 and sequence fragments of a highly divergent feline rotavirus and picobirnavirus. Feline sakobuvirus A represents the prototype species of a proposed new genus in the Picornaviridae family, distantly related to human salivirus and kobuvirus. Feline astroviruses (mamastrovirus 2) are the closest known relatives of the classic human astroviruses (mamastrovirus 1), suggestive of past cross-species transmission. Presence of these viruses by PCR among Portuguese cats was detected in 13% (rotavirus), 7% (astrovirus), 6% (bocavirus), 4% (sakobuvirus), and 4% (picobirnavirus) of 55 feline fecal samples. Co-infections were frequent with 40% (4/10) of infected cats shedding more than one of these five viruses. Our study provides an initial description of the feline fecal virome indicating a high level of asymptomatic infections. Availability of the genome sequences of these viruses will facilitate future tropism and feline disease association studies.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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