Aquaporin-11 control of testicular fertility markers in Syrian hamsters.

Autor: Shannonhouse JL; Institute for Neuroscience, Texas A&M University, College Station, TX 77843, USA., Urbanski HF; Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA., Woo SL; Department of Nutrition & Food Science, Texas A&M University, College Station, TX 77843, USA., Fong LA; Department of Nutrition & Food Science, Texas A&M University, College Station, TX 77843, USA., Goddard SD; Department of Statistics, Texas A&M University, College Station, TX 77843, USA., Lucas WF; Department of Statistics, Texas A&M University, College Station, TX 77843, USA., Jones ER; Department of Statistics, Texas A&M University, College Station, TX 77843, USA., Wu C; Department of Nutrition & Food Science, Texas A&M University, College Station, TX 77843, USA., Morgan C; Institute for Neuroscience, Texas A&M University, College Station, TX 77843, USA; Department of Nutrition & Food Science, Texas A&M University, College Station, TX 77843, USA. Electronic address: camorgan@tamu.edu.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2014 Jun 25; Vol. 391 (1-2), pp. 1-9. Date of Electronic Publication: 2014 Apr 30.
DOI: 10.1016/j.mce.2014.04.011
Abstrakt: The present study sought novel changes to the hamster testicular transcriptome during modulation of fertility by well-characterized photoperiodic stimuli. Transition from long days (LD, 14 h light/day) to short days (SD, 10h light/day) triggered testicular regression (61% reduction of testis weight, relative to LD) in SD-sensitive (SD-S) hamsters within 16 weeks. After 22 weeks of SD exposure, a third cohort of hamsters became SD-refractory (SD-R), and exhibited testicular recrudescence (137% testis weight gain, relative to SD-S). Partial interrogation of the testicular transcriptome by annealing-control-primer-modified differential display PCR provided several candidates for regulation of testicular functions. Multiple linear regression modeling indicated the best correlation for aquaporin 11 (Aqp11) with changes in testis weight. Correlations were also strongest for Aqp11 with expression levels of reference cDNAs that control spermatogenesis (Hspa2 and Tnp2), steroidogenesis (Cox2, 3βHsd, and Srebp2), sperm motility (Catsper1, Pgk2, and Tnp2), inflammation (Cox2), and apoptosis (Bax and Bcl2). Moreover, siRNA-mediated knockdown of testicular Aqp11 mRNA and protein reduced Hspa2 and Tnp2 mRNA levels, and it increased 3βHsd mRNA levels. It also reduced mRNA levels for Sept12, which is a testis-specific inducer of spermatogenesis. These results suggest a central role for testicular Aqp11 signaling in the coordinate regulation of crucial components of fertility.
(Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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