Synergistic effect of anti and pro-inflammatory cytokine genes and their promoter polymorphism with ST-elevation of myocardial infarction.

Autor: Biswas S; Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata 700054, India., Ghoshal PK; Department of Cardiology, N.R.S. Medical College & Hospital, 138 A.J.C. Bose Road, Kolkata 700014, India; Department of Medicine, N.R.S. Medical College & Hospital, 138 A.J.C. Bose Road, Kolkata 700014, India., Mandal N; Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata 700054, India. Electronic address: mandaln@rediffmail.com.
Jazyk: angličtina
Zdroj: Gene [Gene] 2014 Jul 10; Vol. 544 (2), pp. 145-51. Date of Electronic Publication: 2014 Apr 29.
DOI: 10.1016/j.gene.2014.04.065
Abstrakt: Background: The single-gene approach in association studies of polygenic diseases such as acute myocardial infarction (AMI) is likely to provide limited value. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) plasma levels may be genetically influenced.
Aim: We evaluate the impact of single nucleotide polymorphism of the promoter region of these genes, as well as reciprocal interaction of these genes with ST-elevation of myocardial infarction (STEMI).
Methods: In a case-control study 500 STEMI patients and 500 age- and sex-matched controls were studied. Three single-nucleotide polymorphism genotypes were evaluated by polymerase chain reaction and restriction enzyme analysis and assessed their association with STEMI. The synergistic effects of IL-6, TNF-α and IL-10 gene polymorphisms were evaluated by using logistic regression analysis.
Results: We found that IL-6 and TNF-α concentrations of studied population were significantly different (p<0.0001) in each genotype of IL-6 -174G>C and TNF-α -308G>A gene polymorphisms respectively. A significant association was found in multivariate analysis for the IL-6 -174G>C [odds ratio (OR): 0.390; 95% confidence interval (CI): 0.176-0.865, p=0.020] and TNF-α -308G>A [OR: 0.372; 95% CI: 0.171-808, p=0.012] gene polymorphisms with STEMI. In contrast, IL-10 -592C>A gene polymorphism was no longer significant in the multivariate model (OR: 0.678; 95% CI: 0.288 to 1.594, p=0.373) whereas significant in univariate analysis (OR: 0.697; 95% CI: 0.523-0.929, p=0.014).
Conclusions: Our findings suggest that IL-6, TNF-α and IL-10 gene polymorphisms all contribute in the association with STEMI whereas the association persisted only for IL-6 and TNF-α but not for IL-10 gene polymorphism with this disease in the multivariate analysis.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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