[The specific features of the immunophenotype of blast cells in patients with de novo normal karyotype acute myeloid leukemia and FLT3-ITD mutation].

Autor:	Gritsaev SV, Martynkevich IS, Chubukina ZhV, Petrova EV, Kostroma II, Ivanova MP, Martynenko LS, Potikhonova NA, Bubnova LN, Abdulkadyrov KM
Jazyk:	ruština
Zdroj:	Terapevticheskii arkhiv [Ter Arkh] 2014; Vol. 86 (3), pp. 71-7.
Abstrakt:	Aim: To study the specific features of de novo acute myeloid leukemia (AML) with FLT3-ITD mutation. Subjects and Methods: The results of examination were analyzed in 101 patients. Bone marrow morphological specimens were stained with Pappenheim stain. The karyotype was investigated using the standard GTG-banding method. Blast cells were immunotyped in a five-color analysis on a Cytomics FC 500 laser flow cytofluorometer. Results: FLT3-ITD mutation was identified in 21 patients who had a varying morphological nature of blasts, different karyotype variants, and frequently additional NPM1 gene mutation. The distinctive property of 10 patients with normal karyotype and FLT3-ITD mutation (without NPM1 gene mutation) was the larger number of cases with high expression of HLA-DR and CD7 than in the control group that included 18 patients with normal karyotype AML without FLT3-ITD nutation: 50% versus 6.2% (p = 0.007) and 100% versus 55.6% (p = 0.014), respectively. Conclusion: Normal karyotype AML with FLT3-ITD mutation is a group that is homogeneous in the biological phenotype of leukemia cells.
Databáze:	MEDLINE
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