Synergistic activity of the tyrocidines, antimicrobial cyclodecapeptides from Bacillus aneurinolyticus, with amphotericin B and caspofungin against Candida albicans biofilms.

Autor:	Troskie AM; BIOPEP Peptide Group, Department of Biochemistry, Science Faculty, University of Stellenbosch, Stellenbosch, South Africa., Rautenbach M; BIOPEP Peptide Group, Department of Biochemistry, Science Faculty, University of Stellenbosch, Stellenbosch, South Africa., Delattin N; Centre of Microbial and Plant Genetics, CMPG, KU Leuven, Heverlee, Belgium., Vosloo JA; BIOPEP Peptide Group, Department of Biochemistry, Science Faculty, University of Stellenbosch, Stellenbosch, South Africa., Dathe M; Leibniz Institute of Molecular Pharmacology, Berlin, Germany., Cammue BP; Centre of Microbial and Plant Genetics, CMPG, KU Leuven, Heverlee, Belgium Department of Plant Systems Biology, VIB, Ghent, Belgium bruno.cammue@biw.kuleuven.be., Thevissen K; Centre of Microbial and Plant Genetics, CMPG, KU Leuven, Heverlee, Belgium.
Jazyk:	angličtina
Zdroj:	Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2014 Jul; Vol. 58 (7), pp. 3697-707. Date of Electronic Publication: 2014 Apr 21.
DOI:	10.1128/AAC.02381-14
Abstrakt:	Tyrocidines are cationic cyclodecapeptides from Bacillus aneurinolyticus that are characterized by potent antibacterial and antimalarial activities. In this study, we show that various tyrocidines have significant activity against planktonic Candida albicans in the low-micromolar range. These tyrocidines also prevented C. albicans biofilm formation in vitro. Studies with the membrane-impermeable dye propidium iodide showed that the tyrocidines disrupt the membrane integrity of mature C. albicans biofilm cells. This membrane activity correlated with the permeabilization and rapid lysis of model fungal membranes containing phosphatidylcholine and ergosterol (70:30 ratio) induced by the tyrocidines. The tyrocidines exhibited pronounced synergistic biofilm-eradicating activity in combination with two key antifungal drugs, amphotericin B and caspofungin. Using a Caenorhabditis elegans infection model, we found that tyrocidine A potentiated the activity of caspofungin. Therefore, tyrocidines are promising candidates for further research as antifungal drugs and as agents for combinatorial treatment. (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu