Correlation between depression, anxiety, and polymorphonuclear cells' resilience in ulcerative colitis: the mediating role of heat shock protein 70.
| Autor: | Vlachos II; First Department of Pathology, Medical School, Athens University, Athens, Greece. vlachos.ilias@gmail.com., Barbatis C, Tsopanomichalou M, Abou-Assabeh L, Goumas K, Ginieri-Coccossis M, Economou M, Papadimitriou GN, Patsouris E, Nicolopoulou-Stamati P |
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| Jazyk: | angličtina |
| Zdroj: | BMC gastroenterology [BMC Gastroenterol] 2014 Apr 17; Vol. 14, pp. 77. Date of Electronic Publication: 2014 Apr 17. |
| DOI: | 10.1186/1471-230X-14-77 |
| Abstrakt: | Background: To investigate whether anxiety and depression levels are associated with Heat Shock Protein 70 (HSP70) induction in the colon of patients with ulcerative colitis (UC). Methods: The design was cross-sectional. Clinical activity was assessed by the Rachmilewitz Index (CAI). Three psychometric questionnaires were used: Zung Depression Rating Scale (ZDRS), Spielberg State-Trait Anxiety Inventory (STAI), Hospital Anxiety and Depression Scale (HADS). Colon biopsies were obtained from each affected anatomical site. Severity of inflammation was assessed by eosin/hematoxylin. Constitutive (HSP70c) and inducible (HSP70i) HSP70 expression were immunohistochemically studied. Results: 29 UC patients were enrolled (69% men). Mean age was 46.5 years (SD: 19.5). Inflammation severity was moderate in 17 patients, severe in 6, and mild in 6. The mean number of years since diagnosis was 7.9 (SD: 6.5). The mean CAI was 6.4 (SD: 3.1). In active UC, there was downregulation of HSP70c in inflamed epithelium, without significant HSP70 induction. In 22/29 cases of active cryptitis, polymorphonuclear cells (PMN) clearly expressed HSP70i, with weak, focal positivity in the other 7 cases. Except for the hospital anxiety scale, scores in all psychometric tools were higher in patients with strong HSP70i immunoreactivity in the PMN. Logistic regression showed a strong positive relationship between HSP70i immunoreactivity in the PMN cells and scores in the trait anxiety, ZDRS, and hospital depression scales, (Odds ratios 1.3, 1.3, and 1.5; P = 0.018, 0.023, and 0.038; Wald test, 5.6, 5.2, and 4.3 respectively) and a weaker but significant positive correlation with the CAI (Odds ratio 1.654; P = 0.049; Wald test 3.858). Conclusion: HSP70 is induced in PMN cells of UC patients and its induction correlates with depression and anxiety levels. |
| Databáze: | MEDLINE |
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