Molecular typing and characterization of TEM, SHV, CTX-M, and CMY-2 β-lactamases in Enterobacter cloacae strains isolated in patients and their hospital environment in the west of Algeria.

Autor: Souna D; Laboratoire antibiotiques, antifongiques, physico-chimie, synthèse et activité biologiques (LAPSAB), faculté des sciences de la nature, de la vie, de la terre et de l'univers, université Abou-Bekr-Belkaid-Tlemcen, BP 119, Tlemcen 13000, Algeria. Electronic address: souna.microbiologie@yahoo.fr., Amir AS; Service d'anesthésie-réanimation du CHU Hassani-Abdelkader-Sidi, Bel-Abbes, Algeria., Bekhoucha SN; Laboratoire de microbiologie du CHU d'Oran, rue du Dr Benzerdjeb-Oran, Oran, Algeria., Berrazeg M; Laboratoire antibiotiques, antifongiques, physico-chimie, synthèse et activité biologiques (LAPSAB), faculté des sciences de la nature, de la vie, de la terre et de l'univers, université Abou-Bekr-Belkaid-Tlemcen, BP 119, Tlemcen 13000, Algeria., Drissi M; Laboratoire antibiotiques, antifongiques, physico-chimie, synthèse et activité biologiques (LAPSAB), faculté des sciences de la nature, de la vie, de la terre et de l'univers, université Abou-Bekr-Belkaid-Tlemcen, BP 119, Tlemcen 13000, Algeria.
Jazyk: angličtina
Zdroj: Medecine et maladies infectieuses [Med Mal Infect] 2014 Apr; Vol. 44 (4), pp. 146-52. Date of Electronic Publication: 2014 Apr 14.
DOI: 10.1016/j.medmal.2014.01.008
Abstrakt: Objectives: Enterobacter cloacae is a major nosocomial bacterium causing severe infections. A multicenter retrospective cohort study was conducted to collect baseline information on the molecular characteristics of β-lactamase producing Enterobacter cloacae in the west of Algeria.
Materials and Methods: We report a series of 42 extended-spectrum β-lactamase (ESBL) producing non-repetitive Enterobacter cloacae strains, collected in 3 university hospital (Tlemcen, Oran, and Sidi Bel Abbes). Antibiotic susceptibility testing (antibiogram and MIC) and screening for ESBL were performed according to the French Society for Microbiology guidelines. PFGE typing was used to characterize the clonality of all the strains. β-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaECB, and blaCMY-2) were amplified by PCR with specific primers. Plasmid isolation, electroporation, and conjugation experiments were carried out using standard methods.
Results: Sequence analysis revealed that most strains produced CTX-M type ESBLs (CTX-M-15 and CTX-M-3), whereas only 5 produced SHV-type ESBLs (SHV-12). The blaTEM gene was identified in all strains of Enterobacter cloacae. Several epidemic clones were determined. One strain was found to produce plasmid-mediated AmpC β-lactamase (CMY-2); this gene was transferred from E. cloacae by electroporation. Conjugation experiments showed that blaCTX-M, blaTEM, and blaSHV were carried by conjugative plasmids of high molecular weight (≥70kb).
Conclusion: The emergence of resistance genes is a public health problem.
(Copyright © 2014. Published by Elsevier SAS.)
Databáze: MEDLINE
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