Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections.

Autor: Moreno LZ; Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil. luzanolli@gmail.com., Paixão R, Gobbi DD, Raimundo DC, Ferreira TP, Moreno AM, Hofer E, Reis CM, Matté GR, Matté MH
Jazyk: angličtina
Zdroj: Journal of infection in developing countries [J Infect Dev Ctries] 2014 Apr 15; Vol. 8 (4), pp. 416-23. Date of Electronic Publication: 2014 Apr 15.
DOI: 10.3855/jidc.4188
Abstrakt: Introduction: Listeria species are susceptible to most antibiotics. However, over the last decade, increasing reports of multidrug-resistant Listeria spp. from various sources have prompted public health concerns. The objective of this study was to characterize the antibiotic susceptibility of Listeria spp. and the genetic mechanisms that confer resistance.
Methodology: Forty-six Listeria spp. isolates were studied, and their minimal inhibitory concentrations of antibiotics were determined by microdilution using Sensititre standard susceptibility MIC plates. The isolates were screened for the presence of gyrA, parC, lde, lsa(A), lnu(A), and mprF by PCR, and the amplified genes were sequenced.
Results: All isolates were susceptible to penicillin, ampicillin, tetracycline, erythromycin, and carbapenems. Resistance to clindamycin, daptomycin, and oxacillin was found among L. monocytogenes and L. innocua, and all species possessed at least intermediate resistance to fluoroquinolones. GyrA, parC, and mprF were detected in all isolates; however, mutations were found only in gyrA sequences. A high daptomycin MIC, as reported previously, was observed, suggesting an intrinsic resistance of Listeria spp. to daptomycin.
Conclusions: These results are consistent with reports of emerging resistance in Listeria spp. and emphasize the need for further genotypic characterization of antibiotic resistance in this genus.
Databáze: MEDLINE
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