[Retrospective study of the implementation of the qualitative PCR technique in biological samples for monitoring toxoplasmosis in pediatric patients receiving hematopoietic stem cell transplantation].

Autor:	Nigro MG; Departamento de Parasitología, Laboratorio de Toxoplasmosis, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Figueroa C; Servicio de Trasplante de Médula Ósea, Hospital de Pediatría S.A.M.I.C. 'Profesor Dr. Juan P. Garrahan', Ciudad Autónoma de Buenos Aires, Argentina., Ledesma BA; Departamento de Parasitología, Laboratorio de Toxoplasmosis, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: bibilede@anlis.gov.ar.
Jazyk:	Spanish; Castilian
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2014 Jan-Mar; Vol. 46 (1), pp. 24-9.
DOI:	10.1016/S0325-7541(14)70043-4
Abstrakt:	Toxoplasmosis is an opportunistic infection caused by the parasite Toxoplasma gondii. The infection is severe and difficult to diagnose in patients receiving allogeneic hematopoietic stem cell transplantation (HSCT). Twelve patients receiving HSCT were monitored post-transplant, by qualitative PCR at the Children's Hospital S.A.M.I.C. "Prof. Dr. Juan P. Garrahan". The monitoring of these patients was defined by a history of positive serology for toxoplasmosis in the donor or recipient and because their hematologic condition did not allow the use of trimethoprim-sulfamethoxazole for prophylaxis. During the patients' monitoring, two of them with positive PCR results showed signs of illness by T. gondii and were treated with pyrimethamine-clindamycin. In two other patients, toxoplasmosis was the cause of death and an autopsy finding, showing negative PCR results. Four patients without clinical manifestations received treatment for toxoplasmosis because of positive PCR detection. In four patients there were no signs of toxoplasmosis disease and negative PCR results during follow-up. The qualitative PCR technique proved useful for the detection of toxoplasmosis reactivation in HSCT recipients, but has limitations in monitoring and making clinical decisions due to the persistence of positive PCR over time and manifestations of toxicity caused by the treatment. (Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu