Impact of short-time urine freezing on the sensitivity of an established Schistosoma real-time PCR assay.

Autor:	Kenguele HM; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands hkenguele@gmail.com., Adegnika AA; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Nkoma AM; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Ateba-Ngoa U; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Mbong M; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Zinsou J; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Lell B; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands., Verweij JJ; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Jazyk:	angličtina
Zdroj:	The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2014 Jun; Vol. 90 (6), pp. 1153-5. Date of Electronic Publication: 2014 Apr 07.
DOI:	10.4269/ajtmh.14-0005
Abstrakt:	Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR. (© The American Society of Tropical Medicine and Hygiene.)
Databáze:	MEDLINE
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