The age-dependent epigenetic and physiological changes in an Arabidopsis T87 cell suspension culture during long-term cultivation.

Autor: Kwiatkowska A; Department of Botany, University of Rzeszow, Kolbuszowa, Poland. Electronic address: A.Kwiatkows@gmail.com., Zebrowski J; Department of Plant Physiology, University of Rzeszow, Kolbuszowa, Poland., Oklejewicz B; Department of Genetics, University of Rzeszow, Kolbuszowa, Poland., Czarnik J; Department of Botany, University of Rzeszow, Kolbuszowa, Poland., Halibart-Puzio J; Department of Plant Physiology, University of Rzeszow, Kolbuszowa, Poland., Wnuk M; Department of Genetics, University of Rzeszow, Kolbuszowa, Poland.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 May 02; Vol. 447 (2), pp. 285-91. Date of Electronic Publication: 2014 Apr 04.
DOI: 10.1016/j.bbrc.2014.03.141
Abstrakt: Plant cell suspension cultures represent good model systems applicable for both basic research and biotechnological purposes. Nevertheless, it is widely known that a prolonged in vitro cultivation of plant cells is associated with genetic and epigenetic instabilities, which may limit the usefulness of plant lines. In this study, the age-dependent epigenetic and physiological changes in an asynchronous Arabidopsis T87 cell culture were examined. A prolonged cultivation period was found to be correlated with a decrease in the proliferation rate and a simultaneous increase in the expression of senescence-associated genes, indicating that the aging process started at the late growth phase of the culture. In addition, increases in the heterochromatin-specific epigenetic markers, i.e., global DNA methylation, H3K9 dimethylation, and H3K27 trimethylation, were observed, suggesting the onset of chromatin condensation, a hallmark of the early stages of plant senescence. Although the number of live cells decreased with an increase in the age of the culture, the remaining viable cells retained a high potential to efficiently perform photosynthesis and did not exhibit any symptoms of photosystem II damage.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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