[Effect of triiodothyronine on nucleic acid synthesis of immunocytes in cell culture].
| Autor: | Baeva EV |
|---|---|
| Jazyk: | ruština |
| Zdroj: | Problemy endokrinologii [Probl Endokrinol (Mosk)] 1989 Jan-Feb; Vol. 35 (1), pp. 79-83. |
| Abstrakt: | A direct action of T3 on the synthesis of nucleic acids in immunocytes in a cell culture was studied. Scintillation radiometry has shown that T3 makes a regulatory effect on anabolism of nucleic acids in thymocytes and peripheral blood mononuclears at certain stimulating doses of the hormone. T- and B-splenocytes were characterized by metabolic inertness with relation to T3. Serum absence in a culture medium did not prevent the activation of RNA and DNA synthesis in thymocytes. Optimum mitogenic doses of the hormone in a serum-free medium were lower than those in a conditioned culture medium. A study of the kinetics of RNA and DNA synthesis activation by T3 has shown that a regulatory action of the hormone results in the activation of RNA synthesis "triggering". This conclusion is confirmed by evidence on inhibition of G0-G1 transition of the cellular cycle by antiserum to T3. T3 influence on DNA synthesis activation is mediated by a rise of the level of cellular RNA. It produces no direct effect on the G0-G1 phase of the cellular cycle. |
| Databáze: | MEDLINE |
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