Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells.

Autor: Nocca G; Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy., Callà C; Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy., Martorana GE; Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy., Cicillini L; Laboratorio di Patologia Clinica, Ospedale M.G. Vannini, Via dell'Acqua Bullicante, 00177 Rome, Italy., Rengo S; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples 'Federico II', Via G. Pansini 5, 80131 Napoli, Italy., Lupi A; Istituto di Chimica del Riconoscimento Molecolare, C.N.R., c/o Largo Francesco Vito 1, 00168 Rome, Italy., Cordaro M; Istituto di Clinica Odontoiatrica, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy., Luisa Gozzo M; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples 'Federico II', Via G. Pansini 5, 80131 Napoli, Italy., Spagnuolo G; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples 'Federico II', Via G. Pansini 5, 80131 Napoli, Italy.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2014; Vol. 2014, pp. 956579. Date of Electronic Publication: 2014 Feb 12.
DOI: 10.1155/2014/956579
Abstrakt: Unlabelled: Several studies have already demonstrated that the incomplete polymerization of resin-based dental materials causes the release of monomers which might affect cell metabolism. The aim of this study was to investigate the effects of triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, urethane dimethacrylate, and 2-hydroxyethyl methacrylate on (1) cellular energy metabolism, evaluating oxygen consumption rate, glucose consumption, glucose 6-phosphate dehydrogenase activity, and lactate production, and (2) cellular redox status, through the evaluation of glutathione concentration and of the activities of enzymes regulating glutathione metabolism.
Methods: Human pulp cells were used and oxygen consumption was measured by means of a Clark electrode. Moreover, reactive oxygen species production was quantified. Enzymatic activity and glucose and lactate concentrations were determined through a specific kit.
Results: Triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and 2-hydroxyethyl methacrylate induced a decrease in oxygen consumption rate, an enhancement of glucose consumption, and lactate production, whilst glucose 6-phosphate dehydrogenase and glutathione reductase activity were not significantly modified. Moreover, the monomers induced an increase of reactive oxygen species production with a consequent increase of superoxide dismutase and catalase enzymatic activities. A depletion of both reduced and total glutathione was also observed.
Conclusion: The obtained results indicate that dental monomers might alter energy metabolism and glutathione redox balance in human pulp cells.
Databáze: MEDLINE
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