HMGB1 localization during experimental periodontitis.

Autor: Nogueira AV; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil., de Souza JA; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil., de Molon RS; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil., Pereira Eda S; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil., de Aquino SG; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil., Giannobile WV; Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, 1011 N. University Avenue, Ann Arbor, MI 48109-1078, USA., Cirelli JA; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, 2° Andar, Centro, 14801-903 Araraquara, SP, Brazil.
Jazyk: angličtina
Zdroj: Mediators of inflammation [Mediators Inflamm] 2014; Vol. 2014, pp. 816320. Date of Electronic Publication: 2014 Feb 20.
DOI: 10.1155/2014/816320
Abstrakt: Aim: This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) in murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1β and in vivo during ligature- or LPS-induced periodontitis in rats.
Material and Methods: For the in vivo study, 36 rats were divided into experimental and control groups, and biopsies were harvested at 7-30 d following disease induction. Bone loss and inflammation were evaluated. HMGB1 expression was assessed by immunohistochemistry, qPCR, and Western blot.
Results: Significant increases in mPDL HMGB1 mRNA occurred at 4, 8, and 12 h with protein expression elevated by 24 h. HMGB1 mRNA expression in gingival tissues was significantly increased at 15 d in the LPS-PD model and at 7 and 15 d in the ligature model. Immunohistochemical staining revealed a significant increase in the number of HMGB1-positive cells during the experimental periods.
Conclusion: The results show that PDL cells produce HMGB1, which is increased and secreted extracellularly after inflammatory stimuli. In conclusion, this study demonstrates that HMGB1 may be associated with the onset and progression of periodontitis, suggesting that further studies should investigate the potential role of HMGB1 on periodontal tissue destruction.
Databáze: MEDLINE
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