Extracellular polysaccharide production by a strain of Pleurotus djamor isolated in the south of Brazil and antitumor activity on Sarcoma 180.

Autor:	Borges GM; Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., De Barba FF; Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Schiebelbein AP; Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Pereira BP; Departamento de Farmácia, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Chaves MB; Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Silveira ML; Departamento de Farmácia, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Pinho MS; Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Furlan SA; Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Mestrado em Engenharia de Processos, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil., Wisbeck E; Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Mestrado em Engenharia de Processos, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2014 Mar 10; Vol. 44 (4), pp. 1059-65. Date of Electronic Publication: 2014 Mar 10 (Print Publication: 2013).
DOI:	10.1590/S1517-83822014005000019
Abstrakt:	Polysaccharides with medicinal properties can be obtained from fruiting bodies, mycelium and culture broth of several fungus species. This work was carried out in batch culture using a stirred tank reactor with two different initial glucose concentrations (40-50 g/L) and pH values (3.0-4.0) to enhance extracellular polysaccharides production by Pleurotus djamor UNIVILLE 001 and evaluate antitumor effect of intraperitonial administration of Pleurotus djamor extract on sarcoma 180 animal model. According to factorial design, the low pH value (pH 3.0) led to a gain of 1.6 g/L on the extracellular polysaccharide concentration, while glucose concentration in the tested range had no significant effect on the concentration of polysaccharide. With 40 g/L initial glucose concentration and pH 3.0, it was observed that yield factor of extracellular polysaccharide on substrate (YP/S = 0.072) and maximum extracellular polysaccharide productivity (Q(Pmax) = 11.26 mg/L.h) were about 188% and 321% respectively higher than those obtained in the experiment performed at pH 4.0. Under these conditions, the highest values of the yield factor of biomass on substrate (YX/S = 0.24) and maximal biomass productivity (Q(Xmax) = 32.2 mg/L.h) were also reached. In tumor response study, mean tumor volume on the 21th day was 35.3 cm(3) in untreated group and 1.6 cm(3) in treated group (p = 0.05) with a tumor inhibition rate of 94%. These impressive results suggests an inhibitory effect of P.djamor extract on cancer cells.
Databáze:	MEDLINE
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