| Autor: |
Londono C; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, M5S 3E5., Soleas J, Lücker PB, Sathananthan S, Aitchison JS, McGuigan AP |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1202, pp. 37-55. |
| DOI: |
10.1007/7651_2014_76 |
| Abstrakt: |
In many tissues, cells must be aligned for proper function. This alignment can occur at the cellular and/or subcellular (protein/molecular) level. The alignment of cytoskeletal components, in fact, precedes whole cell alignment. A variety of methods exist to manipulate cytoskeletal and whole cell alignment; one of the simplest and most predictable involves seeding adherent cells onto defined substrate topography. We present here two methods to create grooved multiwell plates: one involving microfabrication, which allows for custom design of substrate topography, and a simpler, inexpensive method using commercially available diffraction gratings. We also include methods for manual and automatic quantification of cell alignment. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
