| Autor: |
Bregy L; Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland., Sinues PM, Nudnova MM, Zenobi R |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of breath research [J Breath Res] 2014 Jun; Vol. 8 (2), pp. 027102. Date of Electronic Publication: 2014 Mar 28. |
| DOI: |
10.1088/1752-7155/8/2/027102 |
| Abstrakt: |
On-line analysis of exhaled human breath is a growing area in analytical science, for applications such as fast and non-invasive medical diagnosis and monitoring. In this work, we present a novel approach based on ambient ionization of compounds in breath and subsequent real-time mass spectrometric analysis. We introduce a plasma ionization source for this purpose, which has no need for additional gases, is very small, and is easily interfaced with virtually any commercial atmospheric pressure ionization mass spectrometer (API-MS) without major modifications. If an API-MS instrument exists in a laboratory, the cost to implement this technology is only around [Formula: see text]500, far less than the investment for a specialized mass spectrometric system designed for volatile organic compounds (VOCs) analysis. In this proof-of-principle study we were able to measure mass spectra of exhaled human breath and found these to be comparable to spectra obtained with other electrospray-based methods. We detected over 100 VOCs, including relevant metabolites like fatty acids, with molecular weights extending up to 340 Da. In addition, we were able to monitor the time-dependent evolution of the peaks and show the enhancement of the metabolism after a meal. We conclude that this approach may complement current methods to analyze breath or other types of vapors, offering an affordable option to upgrade any pre-existing API-MS to a real-time breath analyzer. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
