Capsaicin induced apoptosis and gene expression dysregulation of human acute lymphoblastic leukemia CCRF-CEM cells.
| Autor: | Bozok Cetintas V; Department of Medical Biology, Ege University School of Medicine, Izmir, Turkey., Tezcanli Kaymaz B, Aktug H, Oltulu F, Taskiran D |
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| Jazyk: | angličtina |
| Zdroj: | Journal of B.U.ON. : official journal of the Balkan Union of Oncology [J BUON] 2014 Jan-Mar; Vol. 19 (1), pp. 183-90. |
| Abstrakt: | Purpose: Capsaicin, an ingredient of red chili pepper, has possible tumorigenicity/genotoxicity properties. We aimed to determine the effects of capsaicin on the proliferation and gene expression profiles of acute lymphoblastic leukemia (ALL) CCRF-CEM cell line. Methods: Cell viability and IC50 dose was determined by WST cytotoxicity assay. qRT-PCR, immunohistochemical staining and western blot methods were used to determine target genes' expression levels. Apoptosis was evaluated by measuring the caspase-3 activity. Results: Capsaicin inhibited the proliferation of CCRFCEM cells in a dose-dependent manner. Increased mRNA expressions of caspase gene family members, activated caspase-3 and decreased mRNA and protein expression of BCL-2 gene indicated apoptotic response to capsaicin. Moreover capsaicin treatment suppressed significantly the expression of the key cell signaling pathways of KRAS, AKT, GAB2, PTPN11, BRAF, INPP5D, MAPK7. Conclusion: Capsaicin induces apoptosis in CCRF-CEM cells and this response is associated with downregulation of cell signaling pathways. |
| Databáze: | MEDLINE |
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