Investigation of deletion of 22pb in KIR2DS4 gene in a population of southern Brazil.
| Autor: | Marangon AV; Departamento de Ciências Básicas da Saúde, Laboratório de Imunogenética, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, PR 87020-900, Brazil., Visentainer JE, Guelsin GA, Clementino SL, Rudnick CC, de Melo FC, Braga MA, Sell AM |
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| Jazyk: | angličtina |
| Zdroj: | Journal of clinical laboratory analysis [J Clin Lab Anal] 2014 Nov; Vol. 28 (6), pp. 440-5. Date of Electronic Publication: 2014 Mar 22. |
| DOI: | 10.1002/jcla.21707 |
| Abstrakt: | Background: The aim of this study was to investigate the distribution of full-length and deleted variants of KIR2DS4 in a population of southern Brazil and compare the results with other populations, as well as comparing two techniques, PCR-SSP and PCR-SSO, for typing of variants. Methods: 258 individuals from southern Brazil were analysed by PCR-SSO ("polymerase chain reaction-sequence specific oligonucleotides", One Lambda, Inc., Canoga Park, CA), of which 161 were also analysed by PCR-SSP. Results: The study population showed similarities with other Caucasian populations; 46.5% of individuals had only KIR2DS4 variants, 21.3% had the full-length form and 25.1% had both forms. Conclusion: The frequencies found in both groups (genotyped by PCR-SSP and PCR-SSO) were 100% concordant. (© 2014 Wiley Periodicals, Inc.) |
| Databáze: | MEDLINE |
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