Evaluation of storage conditions on equine adipose tissue-derived multipotent mesenchymal stromal cells.

Autor: Mercati F; Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy. Electronic address: francesca.mercati@unipg.it., Pascucci L; Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy., Curina G; Experimental Zooprophylactic Institute of Umbria and Marche, Via G. Salvemini, 1, 06126 Perugia, Italy., Scocco P; School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino (MC), Italy., Tardella FM; School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino (MC), Italy., Dall'aglio C; Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy., Marini C; Experimental Zooprophylactic Institute of Umbria and Marche, Via G. Salvemini, 1, 06126 Perugia, Italy., Ceccarelli P; Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
Jazyk: angličtina
Zdroj: Veterinary journal (London, England : 1997) [Vet J] 2014 May; Vol. 200 (2), pp. 339-42. Date of Electronic Publication: 2014 Feb 27.
DOI: 10.1016/j.tvjl.2014.02.018
Abstrakt: The transplantation of multipotent mesenchymal stromal cells (MSCs) is a potentially promising therapy for the treatment of tendon and ligament injuries and some forms of articular pathology in horses. This study investigated the effects of storage conditions on MSCs. Equine adipose tissue-derived MSCs (eAd-MSCs) were stored at 4 °C and at room temperature (RT) for 24 and 48 h, and viability, doubling time, expression of CD44 and CD90 antigens, clonogenic/differentiation potentials, and karyotype were subsequently evaluated. The eAd-MSC viability was significantly affected by the storage conditions, while doubling time was not significantly altered. The findings indicate (1) that storage at 4 °C is preferable to RT as this results in greater numbers of viable, morphologically normal cells, and (2) that cells should be used within 24 h.
(Copyright © 2014. Published by Elsevier Ltd.)
Databáze: MEDLINE
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