Does women's age influence zona pellucida birefringence of metaphase ΙΙ oocytes in in-vitro maturation program?

Autor: Omidi M; Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran., Khalili MA; Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ; Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran., Nahangi H; Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran., Ashourzadeh S; Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran., Rahimipour M; Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Jazyk: angličtina
Zdroj: Iranian journal of reproductive medicine [Iran J Reprod Med] 2013 Oct; Vol. 11 (10), pp. 823-8.
Abstrakt: Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of PolScope, it has become possible to evaluate zona pellucida (ZP) characteristics as a parameter of oocyte quality. Moreover, quality of oocytes can be influenced by many factors, such as patient's age. The PolScope system is a non-invasive technique to assess birefringent structures such as the meiotic spindle and ZP in living oocytes.
Objective: The aim was to determine the influence of the woman's age on ZP birefringence, a sign of oocyte quality, and morphology of in-vitro matured human oocytes using non-invasive polarized light (PolScope) microscopy.
Materials and Methods: ZP birefringence and morphology were determined in 105 retrieved oocytes from 58 women undergoing ICSI in two age groups (≥30 years and <30 years). The immature oocytes were selected and after IVM, the quality of metaphase ΙΙ (MII) oocytes was assessed. The oocytes abnormalities were classified as intracytoplasmic and extracytoplasmic abnormalities.
Results: Oocyte maturation rates were significantly reduced in ≥30 year's women (56%) in comparison with other age group (80.7%). In addition, the ZP birefringence was significantly higher in MII oocytes in the younger group compared with the older group (76.2% vs. 38.1%; p=0.00). Following morphologic assessment, the rates of oocytes with extracytoplasmic (p=0.02) and both abnormalities (extra- and intracytoplasmic) (p=0.01) were higher in aged versus the younger women.
Conclusion: There was a positive relationship between advanced maternal age with decreased ZP birefringence and oocyte morphological quality in in-vitro matured human oocytes.
Databáze: MEDLINE
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