Probing poly(N-isopropylacrylamide-co-butylacrylate)/cell interactions by atomic force microscopy.

Autor: Natalia B; Department of Informatics Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, Via Opera Pia, 13, Genova, Italy; Grupo Ciencia de Materiales. Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia; Grupo Ingeniería de Tejidos y Terapia celular Facultad de Medicina Laboratorio Terapia celular y Biobanco, IPS Universitaria, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, Colombia., Henry A, Betty L, Marina RL, Roberto R
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2015 Jan; Vol. 103 (1), pp. 145-53. Date of Electronic Publication: 2014 Mar 21.
DOI: 10.1002/jbm.a.35163
Abstrakt: Poly(N-isopropylacrylamide) based hydrogels have been proposed as cell culture supports in cell sheet engineering. Toward this goal, we characterized the poly(N-isopropylacrylamide-co-butylacrylate) copolymer thermo-sensitivity and the cell/copolymer interactions above and below the copolymer lower critical solution temperature. We did that by direct force measurements at different temperatures using an atomic force microscope with either a polystyrene or a glass microbead as probes. We used a copolymer-coated microbead to measure adhesion after a short contact time with a single fibroblast in culture. Statistical analysis of the maximum adhesion force and the mechanical work necessary to separate the probe from the cell surface confirmed the hydrophilic/hydrophobic behavior of poly(N-isopropylacrylamide-co-butylacrylate) as a function of temperature in the range 20-37°C and, consequently, a reversible increase/decrease in cell adhesion with the copolymer. As control experiments we measured interactions between uncoated microbeads with the copolymer hydrogel or cells as well as interaction of the Poly(N-isopropylacrylamide) homopolymer with cells. These results show the potential of an assay based on atomic force microscopy for an in situ and quantitative assessment of cell/substrate interactions and support the use of poly(N-isopropylacrylamide-co-butylacrylate) copolymer as an efficient culture substrate in cell sheet engineering.
(© 2014 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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