The application of CdSe quantum dots with multicolor emission as fluorescent probes for cell labeling.

Autor: Zhao MX; Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004 (China), Fax: (+86) 371-22864665. zhaomeixia2011@henu.edu.cn., Li Y, Zeng EZ, Wang CJ
Jazyk: angličtina
Zdroj: Chemistry, an Asian journal [Chem Asian J] 2014 May; Vol. 9 (5), pp. 1349-55. Date of Electronic Publication: 2014 Feb 24.
DOI: 10.1002/asia.201301692
Abstrakt: Herein, highly luminescent CdSe quantum dots (QDs) with emissions from the blue to the red region of visible light were synthesized by using a simple method. The emission range of the CdSe QDs could be tuned from λ=503 to 606 nm by controlling the size of the CdSe QDs. Two amino acids, L-tryptophan (L-Trp) and L-arginine (L-Arg), were used as coating agents. The quantum yield (QY) of CdSe QDs (green color) with an optimized thickness could reach up to 52 %. The structures and compositions of QDs were examined by using X-ray diffraction (XRD) and transmission electron microscopy (TEM). Optical properties were studied by using UV/Vis and photoluminescence (PL) spectroscopy and a comparison was made between uncoated and coated CdSe QDs. The amino acid-modified β-cyclodextrin (CD)-coated CdSe QDs presented lower cytotoxicity to cells for 48 h. Furthermore, amino acid-modified β-CD-coated green CdSe QDs in HepG2 cells were assessed by using confocal laser scanning fluorescence microscopy. The results showed that amino acid-modified β-CD-coated green CdSe QDs could enter tumor cells efficiently and indicated that biomolecule-coated QDs could be used as a potential fluorescent probe.
(© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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