Human parvovirus B19 in childhood acute lymphoblastic leukaemia in Basrah.
| Autor: | Ibrahem WN; Department of Microbiology, Department of Paediatrics, College of Medicine, University of Basrah, Basrah, Iraq., Hasony HJ; Department of Microbiology, Department of Paediatrics, College of Medicine, University of Basrah, Basrah, Iraq., Hassan JG; Department of Microbiology, Department of Paediatrics, College of Medicine, University of Basrah, Basrah, Iraq. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | JPMA. The Journal of the Pakistan Medical Association [J Pak Med Assoc] 2014 Jan; Vol. 64 (1), pp. 9-12. |
| Abstrakt: | Objective: To investigate the association of human parvovirus B19 infection with the onset of acute lymphoblastic leukaemia and its effect on TEL-AML-1 fusion gene and the presence of mutant P53. Methods: The case-control study was conducted at Basrah Hospital for Paediatrics and Gynaecology, Basrah, Iraq, from May 2009 to April 2010. A total of 100 blood samples were collected from 40 newly diagnosed cases and 60 healthy children to serve as control matched by age and gender. Human parvovirus B19-IgG and anti-P53 antibody were detected by enzyme-linked immunosorbent assay and TEL-AML-1 fusion gene was detected by reverse transcriptase-polymerase chain reaction on extracted ribonucleic acid from fresh blood samples using specified primers. SPSS 15 was used for statistical analysis. Results: A higher proportion of human parvovirus B19-positive cases was found in leukaemic patients (n=19; 47.5%) compared to 12 (20%) in the control group (p<0.05). There was significant association between TEL-AML-1 translocation and human parvovirus-B19 infection as 10 (71.4%) of TEL-AML-1 translocation-positive cases had human parvovirus-B19 IgG. On the other hand, there was no association between such infections and P53 gene mutation in the patients. Conclusion: Human parvovirus-B19 infection is common in the population, with higher prevalence among leukaemic patients with significant association between human parvovirus-B19 and TEL-AML-1 fusion gene in patients of acute lymphoblastic leukaemia. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|