[Evaluation of expression of innate immunity component genes in mice leukocytes under the effect of synthetic ligands in vivo].
| Autor: | Labzhinov PA, Svitich OA, Gankovskaia LV, Zverev VV |
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| Jazyk: | ruština |
| Zdroj: | Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2013 Nov-Dec (6), pp. 76-80. |
| Abstrakt: | Aim: Study the effect of synthetic ligands on the dynamics of TLR9 and BD-2 gene expression in vivo. Materials and Methods: Synthetic ligands were used whose sequence was homologous to DNA and RNA human viruses. At days 1, 7 and 28 blood sampling from BALB/c line mice was carried out. Determination of level of TLR9 and BD-2 gene expression was evaluated by using RT-PCR in real-time mode. Results: The following ligands had the maximum effectiveness: tgg-ccc-ccc-ttg-tgg-acc-gg, ccg-gtc-cac-aag-ggg-ggc-ca and tcg-tcg-ttt-tgt-cgt-tgt-cg. At day 1 after the administration of ligands a significant increase of TLR9 gene expression was observed (62, 18 and 13 times, respectively), but at the same time defensin gene expression changed insignificantly. Conclusion: The approach developed may be used to evaluate the effect of these preparations on the system of innate immunity, namely TLR activation. |
| Databáze: | MEDLINE |
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