Rabies virus glycoprotein and immune response pattern using recombinant protein or recombinant RNA viral vectors.

Autor: Astray RM; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil., Ventini DC; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil., Boldorini VL; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil., Silva FG; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil., Rocca MP; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil., Pereira CA; Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brazil 1500, 05503-900 São Paulo, Brazil. Electronic address: carlos.pereira@butantan.gov.br.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2014 May 19; Vol. 32 (24), pp. 2829-32. Date of Electronic Publication: 2014 Mar 02.
DOI: 10.1016/j.vaccine.2014.02.029
Abstrakt: The present study shows the humoral and cellular aspects of immune response generated by a recombinant rabies virus glycoprotein (rRVGP) as compared to those generated by viral vector carrying the RNA coding for this protein (RVGP-RNA). The rRVGP was synthesized by stably transfected Drosophila melanogaster Schneider 2 (S2) cells and the RVGP-RNA was carried by a recombinant Semiliki Forest Virus (SFV-RVGP). The data show that protein as well as the RNA vaccine was capable of inducing reasonably acceptable levels of antibodies as compared to the optimized commercial whole virus vaccine. As expected, the RNA vaccine was clearly more effective than the protein vaccines in inducing a cellular immune response, as evaluated by the IgG2a/IgG1 ratio and synthesis of interferon gamma (IFNγ) and interleukin 2 (IL2). Our study supports the importance of vaccine designing taking into consideration the concept of DNA/RNA ability to induce an effective cell immune response.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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