Liposomes of phosphatidylcholine and cholesterol induce an M2-like macrophage phenotype reprogrammable to M1 pattern with the involvement of B-1 cells.

Autor: Cruz-Leal Y; Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba., Lucatelli Laurindo MF; Immunology Discipline, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP) Paulista Medicine School, São Paulo 04023-062, São Paulo, Brazil., Osugui L; Immunology Discipline, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP) Paulista Medicine School, São Paulo 04023-062, São Paulo, Brazil., Luzardo Mdel C; Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba., López-Requena A; Research Division, Center of Molecular Immunology (CIM), Havana 11600, Cuba., Alonso ME; Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba., Álvarez C; Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba., Popi AF; Immunology Discipline, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP) Paulista Medicine School, São Paulo 04023-062, São Paulo, Brazil., Mariano M; Immunology Discipline, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP) Paulista Medicine School, São Paulo 04023-062, São Paulo, Brazil., Pérez R; Research Division, Center of Molecular Immunology (CIM), Havana 11600, Cuba. Electronic address: rolando@cim.sld.cu., Lanio ME; Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba. Electronic address: mlanio@fbio.uh.cu.
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2014 Jun; Vol. 219 (6), pp. 403-15. Date of Electronic Publication: 2014 Feb 03.
DOI: 10.1016/j.imbio.2014.01.006
Abstrakt: Macrophages respond to endogenous and non-self stimuli acquiring the M1 or M2 phenotypes, corresponding to classical or alternative activation, respectively. The role of B-1 cells in the regulation of macrophage polarization through the secretion of interleukin (IL)-10 has been demonstrated. However, the influence of B-1 cells on macrophage phenotype induction by an immunogen that suppress their ability to secrete IL-10 has not been explored. Here, we studied the peritoneal macrophage pattern induced by liposomes comprised of dipalmitoylphosphatidylcholine (DPPC) and cholesterol (Chol) carrying ovalbumin (OVA) (Lp DPPC/OVA), and the involvement of B-1 cells in macrophage polarization. Peritoneal cells from BALB/c, B-1 cells-deficient BALB/xid and C57BL/6 mice immunized with Lp DPPC/OVA and OVA in soluble form (PBS/OVA) were analyzed and stimulated or not in vitro with lipopolysaccharide (LPS). Peritoneal macrophages from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA showed an M2-like phenotype as evidenced by their high arginase activity without LPS stimulation. Upon stimulation, these macrophages were reprogrammable toward the M1 phenotype with the upregulation of nitric oxide (NO) and a decrease in IL-10 secretion. In addition, high IFN-γ levels were detected in the culture supernatant of peritoneal cells from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA. Nevertheless, still high levels of arginase activity and undetectable levels of IL-12 were found, indicating that the switch to a classical activation state was not complete. In the peritoneal cells from liposomes-immunized BALB/xid mice, levels of arginase activity, NO, and IL-6 were below those from wild type animals, but the last two products were restored upon adoptive transfer of B-1 cells, together with an increase in IFN-γ secretion. Summarizing, we have demonstrated that Lp DPPC/OVA induce an M2-like pattern in peritoneal macrophages reprogrammable to M1 phenotype after LPS stimulation, with the involvement of B-1 cells.
(Copyright © 2014 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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