Microarray detection of human papilloma virus genotypes among Turkish women with abnormal cytology at a colposcopy unit.

Autor: Cilingir IU; Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey., Bengisu E; Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey., Ağaçfidan A; Department of Microbiology, İstanbul University, İstanbul Medical School,İstanbul, Turkey., Koksal MO; Department of Microbiology, İstanbul University, İstanbul Medical School,İstanbul, Turkey., Topuz S; Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey., Berkman S; Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey., Iyibozkurt AC; Department of Obstetrics and Gynecology, İstanbul University, İstanbul Medical School, İstanbul, Turkey.
Jazyk: angličtina
Zdroj: Journal of the Turkish German Gynecological Association [J Turk Ger Gynecol Assoc] 2013 Mar 01; Vol. 14 (1), pp. 23-7. Date of Electronic Publication: 2013 Mar 01 (Print Publication: 2013).
DOI: 10.5152/jtgga.2013.06
Abstrakt: Objective: There is a well-known association between human papilloma virus (HPV) and cervical neoplasia. The aim of this study was to investigate the types of HPV DNA and to compare the results with colposcopic findings among women with abnormal cytology.
Material and Methods: A series of 76 consecutive women attending the clinic with the usual referral indications (ASC-US or higher in Pap) were examined by the conventional diagnostic tools (PAP smear, colposcopy,punch biopsy) and subjected to HPV testing. For HPV genotyping, we used a commercially avaliable HPV DNA chip (Genomica-CLART) which is a PCR based microarray system.The HPV test detected 35types of HPV (HPV-6/-11/-16/-18/-26/-31/-33/-35/-39/-40/-42/-43/-44/-45/-51/-52/-53/-54/-56/-58/-59/-61/-62/-66/-70/-71/-72/-73/-81/-83/84/-85/-89).
Results: Overall, 44.7% of all patients were HPV positive. HPV was positive in 35%, 51.9%, 77.7% of the ASCUS, LSIL and HSIL groups respectively and HPV 16 was the most prevalent type in all groups. 6 %of patients had mutiple infections. 57.8% of biopsy proven SIL's were HPV positive. The most prevalent HPV type was HPV 16 (54.5%).Colposcopic assessment revealed pathologic findings in 94.7% of biopsy proven SIL cases.
Conclusion: Although it has been reported that the prevalence of HPV in the general population is lower than Western countries, and the prevalence and distribution of genotypes are smilar in patients with abnormal cytology. Further population based studies are needed to determine the prevalance and type distribution of HPV with normal and abnormal cytology in Turkish women. Despite the new technological progress in HPV virion, colposcopy is still very important diagnostic tool in the management of abnormal smears.
Databáze: MEDLINE
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