| Autor: |
Gierut JJ; Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;, Jacks TE, Haigis KM |
| Jazyk: |
angličtina |
| Zdroj: |
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2014 Mar 01; Vol. 2014 (3), pp. 307-9. Date of Electronic Publication: 2014 Mar 01. |
| DOI: |
10.1101/pdb.prot073445 |
| Abstrakt: |
Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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