| Autor: |
Berger LR; Post-Graduate Program in Biological Sciences, Federal University of Pernambuco, Recife 50670420, PE, Brazil. quelberger@hotmail.com., Stamford TC; Department of Tropical Medicine, Center of Health Sciences, Federal University of Pernambuco, Recife 50670420, PE, Brazil. thayzastamford@yahoo.com.br., Stamford-Arnaud TM; Department of Tropical Medicine, Center of Health Sciences, Federal University of Pernambuco, Recife 50670420, PE, Brazil. thayzastamford@yahoo.com.br., de Oliveira Franco L; Center of Biology Sciences, Department of Microbiology, Federal Rural University of Pernambuco, Recife 52171900, PE, Brazil. lucianafranco@terra.com.br., do Nascimento AE; Nucleus of Research in Environmental Science and Biotechnology, Catholic University of Pernambuco, Recife 50050590, PE, Brazil. elesbao@unicap.br., Cavalcante HM; Department of Pharmaceutical Sciences, Federal University of Paraíba-UFPB, João Pessoa 58051-900, PB, Brazil. horacinnammc@yahoo.com.br., Macedo RO; Department of Pharmaceutical Sciences, Federal University of Paraíba-UFPB, João Pessoa 58051-900, PB, Brazil. ruimacedo@ccs.ufpb.br., de Campos-Takaki GM; Nucleus of Research in Environmental Science and Biotechnology, Catholic University of Pernambuco, Recife 50050590, PE, Brazil. galba_takaki@yahoo.com.br. |
| Abstrakt: |
Microbiological processes were used for chitin and chitosan production with Cunninghamella elegans UCP/WFCC 0542 grown in different concentrations of two agro-industrial wastes, corn steep liquor (CSL) and cassava wastewater (CW) established using a 2² full factorial design. The polysaccharides were extracted by alkali-acid treatment and characterized by infrared spectroscopy, viscosity, thermal analysis, elemental analysis, scanning electron microscopy and X-ray diffraction. The cytotoxicity of chitosan was evaluated for signs of vascular change on the chorioallantoic membrane of chicken eggs. The highest biomass (9.93 g/L) was obtained in trial 3 (5% CW, 8% CSL), the greatest chitin and chitosan yields were 89.39 mg/g and 57.82 mg/g, respectively, and both were obtained in trial 2 (10% CW, 4% CSL). Chitin and chitosan showed a degree of deacetylation of 40.98% and 88.24%, and a crystalline index of 35.80% and 23.82%, respectively, and chitosan showed low molecular weight (LMW 5.2 × 10³ Da). Chitin and chitosan can be considered non-irritating, due to the fact they do not promote vascular change. It was demonstrated that CSL and CW are effective renewable agroindustrial alternative substrates for the production of chitin and chitosan. |
