Frequency of Chlamydia trachomatis-specific T cell interferon-γ and interleukin-17 responses in CD4-enriched peripheral blood mononuclear cells of sexually active adolescent females.

Autor: Barral R; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Desai R; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Zheng X; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Frazer LC; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Sucato GS; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Haggerty CL; Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA., O'Connell CM; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Zurenski MA; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA., Darville T; Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA. Electronic address: toni.darville@unc.edu.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2014 Jun; Vol. 103, pp. 29-37. Date of Electronic Publication: 2014 Feb 01.
DOI: 10.1016/j.jri.2014.01.002
Abstrakt: An evaluation of CD4 T cell responses to candidate Chlamydia trachomatis vaccine antigens was conducted in an adolescent female cohort exposed through natural infection to explore antigen immunogenicity and correlation with protection from reinfection. The frequency of peripheral blood CD4 T cell IFN-γ and IL-17 responses to three candidate vaccine antigens, polymorphic membrane protein G (PmpG), F (PmpF), and major outer membrane protein (MOMP), were determined by ELISPOT; responses to chlamydial heat shock protein 60 (HSP60) and to elementary bodies (EB) were included for comparison. Responses of Infected (n=8), Seropositive/Uninfected (n=13), and Seronegative/Uninfected (n=18) participants were compared. The median CD4 IFN-γ response to EB was significantly increased in Infected (P=0.003) and Seropositive/Uninfected (P=0.002) versus Seronegative/Uninfected female subjects. Higher rates of positive IFN-γ responders to EB, PmpF, and MOMP were detected in Seropositive/Uninfected versus Seronegative/Uninfected participants (P=0.021). IL-17 responses were generally low. A positive IFN-γ response to any of the antigens tested was associated with a trend toward a reduced risk of reinfection, although not statistically significant. Among this adolescent cohort, chlamydial-specific CD4 IFN-γ but not IL-17 responses were detected in acutely and previously infected participants and a positive CD4 IFN-γ response was associated with a non-significant reduced risk of reinfection.
(Copyright © 2014. Published by Elsevier Ireland Ltd.)
Databáze: MEDLINE
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