Gene expression based evidence of innate immune response activation in the epithelium with oral lichen planus.

Autor: Adami GR; Department of Oral Medicine and Oral Diagnostics, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA. Electronic address: gadami@uic.edu., Yeung AC; Department of Oral Medicine and Oral Diagnostics, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA., Stucki G; Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA., Kolokythas A; Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA., Sroussi HY; Department of Oral Medicine and Oral Diagnostics, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA., Cabay RJ; Department of Pathology, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA., Kuzin I; Division of Allergy/Immunology and Rheumatology, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA., Schwartz JL; Department of Oral Medicine and Oral Diagnostics, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2014 Mar; Vol. 59 (3), pp. 354-61. Date of Electronic Publication: 2014 Jan 04.
DOI: 10.1016/j.archoralbio.2013.12.010
Abstrakt: Objective: Oral lichen planus (OLP) is a disease of the oral mucosa of unknown cause producing lesions with an intense band-like inflammatory infiltrate of T cells to the subepithelium and keratinocyte cell death. We performed gene expression analysis of the oral epithelium of lesions in subjects with OLP and its sister disease, oral lichenoid reaction (OLR), in order to better understand the role of the keratinocytes in these diseases.
Design: Fourteen patients with OLP or OLR were included in the study, along with a control group of 23 subjects with a variety of oral diseases and a normal group of 17 subjects with no clinically visible mucosal abnormalities. Various proteins have been associated with OLP, based on detection of secreted proteins or changes in RNA levels in tissue samples consisting of epithelium, stroma, and immune cells. The mRNA level of twelve of these genes expressed in the epithelium was tested in the three groups.
Results: Four genes showed increased expression in the epithelium of OLP patients: CD14, CXCL1, IL8, and TLR1, and at least two of these proteins, TLR1 and CXCL1, were expressed at substantial levels in oral keratinocytes.
Conclusions: Because of the large accumulation of T cells in lesions of OLP it has long been thought to be an adaptive immunity malfunction. We provide evidence that there is increased expression of innate immune genes in the epithelium with this illness, suggesting a role for this process in the disease and a possible target for treatment.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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