| Autor: |
Bergdale TE; Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, 501 East Saint Joseph Street, Rapid City, SD, 57701, USA., Hughes SR, Bang SS |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2014 Apr; Vol. 172 (7), pp. 3488-501. Date of Electronic Publication: 2014 Feb 19. |
| DOI: |
10.1007/s12010-014-0784-7 |
| Abstrakt: |
A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including β-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 °C. The kinetic constants, K m and V max, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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