Molecular pathology and histopathological findings in localized leishmania lymphadenitis.

Autor: Dabiri S; 1)Pathology Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.6)Leishmaniasis Research Center, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Safavi M; 1)Pathology Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. 7)Clinical Research Unit, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran., Shamsi Meymandi S; Dermatology Department, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran., Yousefi K; Surgery Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Shamsi Meymandi M; Physiology and Pharmacology Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Fotouhi Ardakani R; 8)Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran. 9)Department of Biotechnology, Shahid-Sadoughi University of Medical Sciences, Yazd, Iran., Soofi Abadi MF; Stem Cell Research Center of Pathology Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Jazyk: angličtina
Zdroj: Archives of Iranian medicine [Arch Iran Med] 2014 Feb; Vol. 17 (2), pp. 122-6.
DOI: 014172/AIM.008
Abstrakt: Background: A rare variant of Leishmaniasis is Localized Leishmania Lymphadenitis which has been occasionally reported from south-eastern parts of Iran. So far, no molecular assay has been performed for diagnosing this variety of Leishmaniasis.
Methods: Nineteen lymph node paraffin blocks were collected from 1994 to 2007. Parasite load count and histopathological patterns reported on Hematoxylin-Eosin and Giemsa stained slides.DNA extraction was carried out just on the remaining available 7 lymph node paraffin blocks according to QIAamp DNA FFPE kit instructions. A pair of primers and a probe were designed for rRNA ITS region with Allele ID 6.0 software, followed by real time PCR amplification.
Result: The most common histopathological pattern was necrotizing granuloma with few Leishman bodies. Parasite load was the highest in submental lymph node (3 ± 1.41 per oil field) which was significantly higher compared to cervical and inguinal nodes (P < 0.05). Absolute load of parasite DNA was detectable in all 7 cases. The positive cases revealed a 201 bpamplicon after electrophoresis of end product which was confirmative for Leishmania tropica.
Conclusion: Real time PCR revealed Leishmania tropica as the etiologic agent of Localized Leishmania Lymphadenitis. Although this molecular method is a sensitive diagnostic tool, histopathological findings are still important.
Databáze: MEDLINE
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